Exercícios de Cinética ENZIMÁTICA

Exercícios de Cinética ENZIMÁTICA

Cintica Ezimtica Exerccios Resolvidos Analisando a cintica enzimtica: Construo dos grficos e

determinao de Km e Vmx. Anlise grfica de Vmx e Km

Primeiro grfico: Vo x [S] grfico a partir dos valores da tabela Vmx. Vmx

Km Segundo Grfico: inverso do anterior Grfico a partir dos valores inversos da

tabela Achando a Velocidade Mxima e o Km Depois de encontrar a equao da reta, possivel calcular a Vmx e o Km atravs

dos valores de X e Y: Y = 3,768x + 2,0993 (Equao da reta) -0,557 quando Y = 0 e 2,099 quando X =0 Exerccio 10 do cap.12 (Voet.2ed.)

Calcule Km e a Velocidade Mxima a partir dos seguintes dados: Primeiro passo: construir o grfico de Lineweaver-

Burk Segundo passo: Regresso Linear (ou extraplotao da reta) Grfico de Lineweaver-Burk

Clculos para achar Km e Vmx.: Achando a equao da reta: y = 0,2152x + 0,7971

x= -3,703 y= 0,7971 p/ y =0 p/ x =0

Vmx = 1,25 Km = 0,27

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